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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度:
0.131 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人早期生长应答因子3(EGR3)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human EGR3(Early Growth Response Protein 3) ELISA Kit |
| 别名 | Early growth response protein 3;EGR-3;Zinc finger protein pilot;PILOT |
| 货号 | ELK11447 |
| 反应种属 | Human |
| Q06889 | Q06889 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.131 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGR3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EGR3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EGR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGR3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Cardiovascular biology; |

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相关专题 酶联免疫斑点法(ELISpot) 在免疫学 领域中,对于疾病以及疫苗研究不仅仅局限于体液免疫应答(B细胞免疫),细胞介导的免疫应答(cell mediated immune response,CMI)也是人们所关注的,而T细胞在CMI中起关键作用。在研究免疫应答机制时以往常用用酶联免疫吸附法(ELISA)检测体液中游离的细胞因子(CK)或抗体,但由于游离的循环抗体或CK的半哀期不同,使之在体液中不断的被代谢或与靶器官结合
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