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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
31.25-2000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
11.3 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 人二氢嘧啶酶样蛋白3(DPYSL3)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human DPYSL3(Dihydropyrimidinase Like Protein 3) ELISA Kit |
| 别名 | Collapsin response mediator protein 4; CRMP 4 ; CRMP-4; CRMP4; Dihydropyrimidinase like 3 ; Dihydropyrimidinase related protein 3; Dihydropyrimidinase-related protein 3; DPYL3; DPYSL3; DRP 3 ; DRP-3; DRP3; LCRMP; TUC4; ULIP 1; ULIP; ULIP-1; ULIP1; UNc 33 like phosphoprotein 1; Unc 33 like phosphoprotein; Unc-33-like phosphoprotein 1 |
| 货号 | ELK11345 |
| 反应种属 | Human |
| Q14195 | Q14195 |
| 检测类型 | Sandwich |
| 灵敏度 | 11.3 pg/mL |
| 标准品 | 2000 pg/mL |
| 检测范围 | 31.25-2000 pg/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DPYSL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DPYSL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DPYSL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DPYSL3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Neuroscience; |

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文献和实验【原创】Protein A Sepharose 之进化篇。。。
[img][/img]Protein A 柱之进化 Protein A是一种金黄色葡萄球菌细胞壁蛋白质,能特异性地与人和哺乳动物抗体(主要是IgG)的Fc区结合。因而,将Protein A 与琼脂糖凝胶以一定的方式结合,可制备用于抗体纯化的亲和填料。 早期的Protein A柱结合的都是天然Protein A。天然Protein A由5个IgG结合域和其它未知功能的非Fc结合域组成,分子量约42KD,结构如图1所示。这种柱子对IgG的亲和能力很强,可以吸附大量的lgG。但同时,天然
对照。然后将这些平板干燥并密封保存在放有干燥剂的铝箔袋中。室温保存一段时间后,单克隆抗体包被的平板被用来进行夹心法酶联免疫测定,而多克隆抗体包被的平板则进行以辣根过氧化物酶为抗原的直接吸附测定。图1 单克隆抗体在室温下稳定性的研究在室温下保存18月后,除了以BSA作为稳定剂的单克隆抗体外,所有单克隆抗体样本的活性回收率都在比较好的范围70-80%(见图1);而多克隆抗体的实验结果则表现出更显著的差异(见图2)。但是,关系到稳定剂Superblock的50%活性的损失,似乎发生在干燥的过程中,而在其后的保存过程中
-8878.3. Takayama, H. et al. 2008. High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS ONE 3: e2314 (DOI: 10.1371/journal.pone.0002314).4. Zhong, M. and Molday, R.S. 2010
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