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- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluidstissue homogenates
- 灵敏度:
0.135 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1680.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2400.0 |
| 中文名称 | 小鼠肾损伤分子1(Kim1)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse Kim1(Kidney Injury Molecule 1) Microsample ELISA Kit |
| 别名 | HAVCR1; TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1 |
| 货号 | ELK11066MS |
| 反应种属 | Mouse |
| Q5QNS5 | Q5QNS5 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.135 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluidstissue homogenates |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Kim1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Kim1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Kim1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Kim1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Tumor immunity;Infection immunity;Kidney biomarker; |

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文献和实验相关实验
phosphate、glycerol、glacial acetic acid、methanol、strerile H2O、chamber slide (Nunc 177380)或chamber slide替代物:35 or 60mm sterile plate内放置无菌22x22mm盖玻片﹙浸于酒精内,使用前过火灭菌﹚,一般吸附型细胞均能吸附在盖玻片上。染色后取出盖玻片,细胞面朝下放在玻片上观察。 · Indicator cells: Vero (African green monkey kidney
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小鼠肾损伤分子1(Kim1)微量上样酶联免疫吸附检测试剂盒【Mouse Kim1(Kidney Injury Molecule 1) Microsample ELISA Kit】
¥1680 - 2400





