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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.13 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1680.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2400.0 |
| 中文名称 | 人趋化因子C-C-基元受体5(CCR5)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Human CCR5(Chemokine C-C-Motif Receptor 5) Microsample ELISA Kit |
| 别名 | CD195; CC-CKR5; CCCKR5; CMKBR5; CHEMR13; HIV-1 fusion coreceptor |
| 货号 | ELK10592MS |
| 反应种属 | Human |
| P51681 | P51681 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.13 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CCR5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCR5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CCR5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCR5 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | CD & Adhesion molecule;Tumor immunity;Infection immunity; |

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文献和实验The CCR5 gene encodes a cell-surface chemokine receptor molecule, which serves as a coreceptor for macrophage-tropic strains of HIV-1 (1 –3 ). Mutations in this gene may alter expression or function of the protein product, thereby altering
sis 的产物是一种分泌蛋白。 1983 年 Waterfeld M.D. 等发现了血小板生长因子( PDGF )与猿猴肉瘤病毒的推断转化蛋白 p28 具有结构的相似性。 Doolittle 等进一步确定 v-sis 癌基因来源于 PDGF 基因。它们之间有 87% 相同。后来发现 c-p28c-sis 与 PDGF 顺序只有 3% 的差异。而人类 c-sis 基因产物则与 PDGF- β链 完全相同。表明病毒癌基因是来源于动物和人类
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人趋化因子受体5 (CCR5)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 CCR5 单抗包被于酶标板上,标准品和样品中的 CCR5与单抗结合,加入生物素化的抗人 CCR5
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