- ￥1820 - 2600
- ELK Biotechnology
- 1.25-80 ng/mL
- 0.49 ng/mL
- 武汉
- ELK0665
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
1.25-80 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Rat
- 样本：
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
0.49 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 大鼠丝裂原活化蛋白激酶(MAPK)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat MAPK(P38 Mitogen Activated Protein Kinase) ELISA Kit |
| 别名 | P38 Mitogen-Activated Protein Kinase |
| 货号 | ELK0665 |
| 反应种属 | Rat |
| 检测类型 | Sandwich |
| 灵敏度 | 0.49 ng/mL |
| 标准品 | 80 ng/mL |
| 检测范围 | 1.25-80 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MAPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MAPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MAPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MAPK in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Enzyme & Kinase;Tumor immunity;Infection immunity; |
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文献和实验Mitogen-Activated Protein Kinase Assays
-activated protein kinases (MAPKs) and their activation kinase cascades, which transduce signals from the plasma membrane to the cytosol and nucleus, are an integral part of signaling pathways involved in many cellular responses. PMN express several members
Analysis of Mitogen-Activated Protein Kinase Activation
signal-regulated kinases, JNK, and p38MAPK also play a significant role in the regulation of mast cell biological function. This chapter will detail recent advances in determining mitogen-activated protein kinase activation in single cells. These methods
Activation of the Mitogen-Activated Protein Kinase Pathway by TGF
The mitogen-activated protein kinase (MAPK) superfamily includes three major subfamilies: extracellular signal-regulated kinase (Erks), c-Jun-N-terminal kinases (JNKs)/stress-activated protein kinases (SAPKs), and p38 kinases (1 –
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