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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Tissue homogenates and other biological fluids.
- 灵敏度:
0.067 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人泛素特异性肽酶18(USP18)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human USP18(Ubiquitin Specific Peptidase 18) ELISA Kit |
| 别名 | 43 kDa ISG15-specific protease (hUBP43);ISG15-specific-processing protease;Ubl thioesterase 18 |
| 货号 | ELK9977 |
| 反应种属 | Human |
| Q9UMW8 | Q9UMW8 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.067 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human USP18. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human USP18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human USP18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human USP18 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase; |

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文献和实验by adding 100 µl of 1M Phosphoric Acid. Read at 450 nm. Conjugate Preparation 1. Centrifuge 0.3ml enzyme (alkaline phosphatase, Sigma type VII, sp act. 1,140 U/ml) at 8,000 x g for 2 mins. 2. Add to the pellet 0.1 ml specific antibody
• 洗板一定力求干净,以保证实验成功。 图 9-1 免疫酶联吸附实验( ELISA )的实验原理 图 9-2 定性 ELISA 实验结果,左为阳性结果,右为阴性结果。
酶免疫测定(enzyme immunoassay, EIA)或免疫酶技术(immunoenzymatic technique)是指用酶标记抗体或酶标记抗体进行的抗原抗体反应。它采用抗原与抗体的特异反应与酶连接,然后通过酶与底物产生颜色反应,用于定量测定。目前常用的方法称为酶联免疫吸附法(enzyme-linked immunosorbent assay, ELISA),其方法简单,方便讯速,特异性强。ELISA是由抗原(抗体)先结合在固相
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