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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
1.57-100 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Rabbit
- 样本:
Serum, plasma and other biological fluids.
- 灵敏度:
0.74 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 兔脂联素受体1(ADIPOR1)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rabbit ADIPOR1(Adiponectin Receptor 1) ELISA Kit |
| 别名 | ACDCR1; CGI45; PAQR1; TESBP1A; TESBP1-A; Progestin and AdipoQ Receptor Family Member I |
| 货号 | ELK9797 |
| 反应种属 | Rabbit |
| 检测类型 | Sandwich |
| 灵敏度 | 0.74 ng/mL |
| 标准品 | 100 ng/mL |
| 检测范围 | 1.57-100 ng/mL |
| 样本类型 | Serum, plasma and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit ADIPOR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit ADIPOR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit ADIPOR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit ADIPOR1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Metabolic pathway; |

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文献和实验的酶活力直接反映游离的酶标记物。均相EIA在临床检验中较少应用。非均相EIA需先进行游离的和结合的标记物的分离。如前所述,固相载体可用作一种分离手段。这种固相酶免疫测定方法在1971年最初建立时称为酶联免疫吸附剂测定(enzyme linked immunosorbent assay),简称ELISA,在国内有译作酶联免疫吸附试验或酶标,已习用。2、ELISA的原理和类型2.1. ELISA的原理ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫
50 median effective dose 半数有效剂量 EDTA ethylendiaminotetraacetic acid 乙二氨四乙酸 EK epidermal keratin 表皮角蛋白 ELISA enzyme-liked immunosorbent assay 酶联免疫吸附分析 EM electron microscope 电镜 ENK enkephalin 脑啡肽 L-ENA (L-NK) Leu-enkephalin 亮氨酸
,平均批间变异系数为11.4%;液相芯片与ELISA法检测结果的相关系数R2为0.88~0.99。 国外多家公司已推出了商品化的细胞因子液相芯片检测试剂盒,比如R&D 公司的Flurokine MAP系列、Linco Research公司的LINCOplex 试剂盒、BioSource公司的Antibody Bead Kit、Bio Rad公司的Bio Plex系列等,可用于来自人、小鼠、大鼠的样品,既有一次检测单种细胞因子的试剂盒,也有预先混合多种细胞因子抗体交联微球而一次检测多个指标
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