人雷帕霉素靶蛋白(mTOR)微量上样酶联免疫吸附检测试剂盒【
Human mTOR(MammalianTarget of Rapamycin) Microsample ELISA Kit】- ￥1820 - 2600
- ELK Biotechnology
- 0.16-10 ng/mL
- 0.1 ng/mL
- 武汉
- ELK9591MS
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉益普生物科有限公司
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
0.1 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 人雷帕霉素靶蛋白(mTOR)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Human mTOR(MammalianTarget of Rapamycin) Microsample ELISA Kit |
| 别名 | FRAP1; RAFT1; RAPT1; Mechanistic target of rapamycin; FK506 binding protein 12-rapamycin associated protein 1 |
| 货号 | ELK9591MS |
| 反应种属 | Human |
| P42345 | P42345 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.1 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human mTOR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human mTOR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human mTOR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human mTOR in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Cell proliferation |
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文献和实验The mammalian target of rapamycin (mTOR) is the catalytic subunit of two multiprotein complexes, mTOR complex-1 (mTORC1) and mTOR complex-2 (mTORC2). Clinically used rapamycin and rapalogs are FKBP12-dependent allosteric inhibitors of mTORC
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠 雷帕霉素靶蛋白 (mTOR)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 mTOR 单抗包被于酶标板上，标准品和样品中的 mTOR 与单抗结合，加入生物素化的抗大鼠 mTOR ，形成免疫复合物连接在板上
Expanding Human T Regulatory Cells with the mTOR-Inhibitor Rapamycin
increasing momentum. However, given the limited amount of circulating FOXP3+ Treg cells, efficient methods for their ex vivo expansion are highly desirable. Rapamycin allows for in vitro expansion of murine and human FOXP3+ Treg cells, which maintain
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