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- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
78.13-5000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
serum, plasma, tissue homogenates, saliva, cell lysates, follicular fluid and other biological fluids
- 灵敏度:
29 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1680.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2400.0 |
| 中文名称 | 大鼠可溶性fms样酪氨酸激酶1(sFLT)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat sFLT(Soluble Fms-Like Tyrosine Kinase Receptor 1) ELISA Kit |
| 别名 | Flt1; Fms-Related Tyrosine Kinase 1; Vascular Permeability Factor Receptor; Fms-like tyrosine kinase 1; Tyrosine-protein kinase receptor FLT |
| 货号 | ELK9331 |
| 反应种属 | Rat |
| 检测类型 | Sandwich |
| 灵敏度 | 29 pg/mL |
| 标准品 | 5000 pg/mL |
| 检测范围 | 78.13-5000 pg/mL |
| 样本类型 | serum, plasma, tissue homogenates, saliva, cell lysates, follicular fluid and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat sFLT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat sFLT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat sFLT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat sFLT in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Tumor immunity; |

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文献和实验is the specificity of the antibody-antigen interaction because monoclonal antibodies bind to only one epitope of the antigen. If the antigen of interest is tyrosine phosphorylated, and this tyrosine phosphorylation is under investigation, each step has to be handled
In Vivo Rat Model of Preeclampsia
and placental growth factor) signaling and endothelial health may play a major role in the pathogenesis of preeclampsia. Exogenous administration of soluble fms-like tyrosine kinase-1 (sFlt-1; a circulating anti-angiogenic protein) results in a preeclampsia
-like tyrosine kinase-3, which is mutated in majority of cases of acute myeloid leukemia, is used as an example. This method could be applied to the quantitation of several other proteins without the need for intact cells.
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