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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
31.25-2000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
13 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 人SATB同源框蛋白(SATB2)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human SATB2(Special AT-rich sequence-binding protein 2) ELISA Kit |
| 别名 | DNA-binding protein SATB2; SATB2; KIAA1034; Special AT-rich sequence-binding protein 2 |
| 货号 | ELK9215 |
| 反应种属 | Human |
| Q9UPW6 | Q9UPW6 |
| 检测类型 | Sandwich |
| 灵敏度 | 13 pg/mL |
| 标准品 | 2000 pg/mL |
| 检测范围 | 31.25-2000 pg/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SATB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SATB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SATB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SATB2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction; |

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文献和实验A Collection of Plant-Specific Genomic Data and Resources at NCBI
to be searched to produce a display of aligned maps from several species. Customized Plant Basic Local Alignment Search Tool (PlantBLAST) allows the user to perform sequence similarity searches in a special collection of mapped plant sequence data and to view
General Advice PCR allows the production of more than 10 million copies of a target DNA sequence from only a few molecules. The sensitivity of this technique means that the sample should not be contaminated with any other DNA or previously amplified
the cDNA towards the 3' ends of transcripts. In my case, I knew that the region I was amplifying should be at the extreme 3' end of the coding sequence, so I used oligo-dT primed cDNA. For the lazy and rich, Clontech sells oligo-dT primed cDNA prepared
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