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- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 灵敏度:
0.061 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 小鼠补体成分C1ra酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse C1ra(Complement Component C1ra) ELISA Kit |
| 别名 | C1ra; C1rComplement C1r-A subcomponent; EC 3.4.21.41; Complement component 1 subcomponent r-A) [Cleaved into: Complement C1r-A subcomponent heavy chain; Complement C1r-A subcomponent light chain] |
| 货号 | ELK8893 |
| 反应种属 | Mouse |
| 检测类型 | Sandwich |
| 灵敏度 | 0.061 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse C1ra. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse C1ra. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse C1ra, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse C1ra in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Immune molecule; |

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Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
between chromatin elements without specifically identifying the protein components that mediate such interactions. Here we describe a detailed protocol for C ombined 3C -C hIP-C loning (6C) technology, which combines multiple techniques to identify the proteins
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