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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
Serum, plasma and other biological fluids.
- 灵敏度:
0.055 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 小鼠肝型脂肪酸结合蛋白(FABP1)酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse FABP1(Fatty Acid Binding Protein 1, Liver) ELISA Kit |
| 别名 | FABP-1; FABPL; L-FABP; LFABP; Liver-type fatty acid-binding protein |
| 货号 | ELK8660 |
| 反应种属 | Mouse |
| P05202 | P05202 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.055 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Serum, plasma and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FABP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FABP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FABP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FABP1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Metabolic pathway; |

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文献和实验近期,来自广西大学动物科技学院的李一星教授和广西壮族自治区人民医院的周磊教授团队在 Imeta 杂志发表了题为「HLF and PPARα axis regulates metabolic‐associated fatty liver disease through extracellular vesicles derived from the intestinal microbiota」的科研论文,IF = 33.2。 研究背景: 代谢相关性脂肪肝(Metabolic
的,但是这结果是不是假阳性呢?还得做个阴性对照。阴性对照如何设置呢?考虑到整个实验体系会出现 beads、抗X抗体、诱饵蛋白 X、靶蛋白 Y(co-IP): 可能出现的假阳性来源 需要的阴性对照 抗 X 抗体结合非特异的蛋白 与 IP 一抗种属亚型相同的免疫球蛋白(比如,抗 X 抗体是 Mouse IgG,阴性对照可使用 Mouse 免疫球蛋白) beads 对蛋白的非特异结合* 同上,此外还可进行 Pre clear,IP 正式实验前排除非特异结合蛋白。如果使用琼脂
功能障碍有关 2024 年 2 月 9 日,南京大学医学院附属鼓楼医院心脏科的研究团队在 Cardiovascular Diabetology(影响因子:9.6)期刊发表了题为:Circulating exosomal mir-16-2-3p is associated with coronary microvascular dysfunction in diabetes through regulating the fatty acid degradation of endothelial cells 的研究
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