小鼠母系胚胎亮氨酸拉链蛋白激酶(MELK)酶联免疫吸附检测试
剂盒【Mouse MELK(Maternal Embryonic Leucine Zipper Kinase) ELISA Kit】- ￥1960 - 2800
- ELK Biotechnology
- 0.16-10 ng/mL
- 0.057 ng/mL
- 武汉
- ELK6956
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉益普生物科有限公司
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Mouse
- 样本：
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度：
0.057 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1960.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2800.0 |
| 中文名称 | 小鼠母系胚胎亮氨酸拉链蛋白激酶(MELK)酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse MELK(Maternal Embryonic Leucine Zipper Kinase) ELISA Kit |
| 别名 | hPK38; Protein kinase Eg3; Tyrosine-protein kinase MELK |
| 货号 | ELK6956 |
| 反应种属 | Mouse |
| Q61846 | Q61846 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.057 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MELK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MELK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MELK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MELK in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase; |
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文献和实验Methods for Analysis of Brain Tumor Stem Cell and Neural Stem Cell Self-Renewal
similar mechanisms and pathways for proliferation. For example, we have identified that one of the AMPK/snf1 kinases, maternal embryonic leucine zipper kinase (MELK), is highly expressed in NSC and malignant brain tumors, as well as in brain tumor stem
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
【分享】使用韩国BIONEER荧光定量PCR仪发表的文章 （50篇）
and Mitsuhoro Yanagida. J Cell Biol. 2008 Mar 24; 180(6):1115-1131. 影响因子：9.12 2 A novel culture technique for human embryonic stem cells using porous membranes Sinae Kim, Seong Eun Ahn, Jae Ho Lee, Do-Seon Lim, Kwang-Soo Kim, Hyung-Min Chung and Soo
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