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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度:
0.068 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人β-1,3-N-乙酰葡糖胺基转移酶3(b3GNT3)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human b3GNT3(Beta-1,3-N-Acetylglucosaminyltransferase 3) ELISA Kit |
| 别名 | B3GAL-T8; B3GN-T3; B3GNT-3; TMEM3; beta3Gn-T3; Putative Type II Membrane Protein; Transmembrane Protein 3; Core 1 extending beta-1,3-N-acetylglucosaminyltransferase |
| 货号 | ELK5071 |
| 反应种属 | Human |
| 检测类型 | Sandwich |
| 灵敏度 | 0.068 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human b3GNT3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human b3GNT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human b3GNT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human b3GNT3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase; |

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文献和实验Study of the Biological Functions of Mucin Type Core 3 O-glycans
Core 3 O -glycan is very short glycan structure which is composed of one N -acetylglucosamine and one N -acetylgalactosamine. The core 3 O -glycan structure is synthesized by core3 synthase (beta 1, 3-N -acetylglucosaminyltransferase 6) using
胞核蛋白的 O-GlcNAc 糖基化。这种在蛋白质上添加 O-GlcNAc 的修饰,在真核生物中非常普遍,发生在丝氨酸或苏氨酸的 β-连接上。尽管序列 Tyr-Ser-Pro-Tb- Ser- Pro -Sef ( 标有 * 是发生糖基化的氨基酸)代表一类典型的 GlcNAc 连接位点 [9] ,但是对可被 OGlcNAc 糖基化的保守序列还不十分清楚。OGlcNAc 糖基化是一种高度动态过程,而且通常与 O-磷酸化修饰交互进行 [10] 。糖基转移酶的催化作用下,糖基被添加到蛋白
原核生物,均为单细胞生物。一般以二分裂的方式繁殖,也有的产生孢子。 一、细菌 是在自然界分布最广、个体数量最多的有机体。 可分为:球菌、杆菌和螺旋菌(弧形菌)。 绝大多数细菌的直径在0.5~5μm之间。 1、细胞壁:厚度一般15-30nm。主要成分是肽聚糖,由N-乙酰葡糖胺和N-乙酰胞壁酸构成双糖单元,以β(1-4)糖苷键连接成大分子。N-乙酰胞壁酸分子上有四肽侧链,相邻聚糖纤维之间的短肽通过肽桥(革兰氏阳性菌)或肽键(革兰氏阴性菌)桥接起来,形成了肽聚糖片层,像胶合板一样,粘合成多层
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