- ￥1820 - 2600
- ELK Biotechnology
- 0.32-20 ng/mL
- 0.118 ng/mL
- 武汉
- ELK3456
- 2026年05月21日
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- 文献和实验
- 技术资料
- 供应商：
武汉益普生物科有限公司
- 检测范围：
0.32-20 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
tissue homogenates, cell lysates and other biological fluids
- 灵敏度：
0.118 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 人线粒体转录因子A(TFAM)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human TFAM(Transcription Factor A, Mitochondrial) ELISA Kit |
| 别名 | MtTF1; TCF6; TCF6L2; mtTFA; Mitochondrial transcription factor 1; Transcription factor 6-like 2 |
| 货号 | ELK3456 |
| 反应种属 | Human |
| Q00059 | Q00059 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.118 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TFAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TFAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TFAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TFAM in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction; |
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文献和实验Methods for Assessing Binding of Mitochondrial Transcription Factor A (TFAM) to DNA
It is now recognized that mammalian mitochondrial DNA forms a higher structure called the nucleoid, corresponding to the nucleosome of nuclear DNA. Mitochondrial transcription factor A (TFAM), which was cloned as a transcription factor
Breeding and Genotyping of Tfam Conditional Knockout Mice
Respiratory chain dysfunction is an important contributor to human pathology (1 –3 ). The generation of animal models has much facilitated in-depth studies of pathogenetic mechanisms in mitochondrial disease (4 ). The function
PGC-1α、TFAM以及OXPHOS相关蛋白的表达水平。 八、线粒体形态：看网络是延长、连接还是碎片化 线粒体形态可直观体现线粒体网络的结构状态。可采用MitoTracker染色、TOM20 免疫荧光，或是线粒体靶向荧光蛋白开展成像，后续借助 ImageJ/Fiji 软件完成定量分析。 常见量化参数包括： 单个线粒体长度 纵横比（Aspect ratio） 线粒体占据面积（Mitochondrial footprint） 分支长度（Branch length
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