- ￥1820 - 2600
- ELK Biotechnology
- 0.32-20 ng/mL
- 0.118 ng/mL
- 武汉
- ELK3456
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉益普生物科有限公司
- 检测范围：
0.32-20 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
tissue homogenates, cell lysates and other biological fluids
- 灵敏度：
0.118 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 人线粒体转录因子A(TFAM)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human TFAM(Transcription Factor A, Mitochondrial) ELISA Kit |
| 别名 | MtTF1; TCF6; TCF6L2; mtTFA; Mitochondrial transcription factor 1; Transcription factor 6-like 2 |
| 货号 | ELK3456 |
| 反应种属 | Human |
| Q00059 | Q00059 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.118 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TFAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TFAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TFAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TFAM in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction; |
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文献和实验Methods for Assessing Binding of Mitochondrial Transcription Factor A (TFAM) to DNA
It is now recognized that mammalian mitochondrial DNA forms a higher structure called the nucleoid, corresponding to the nucleosome of nuclear DNA. Mitochondrial transcription factor A (TFAM), which was cloned as a transcription factor
Breeding and Genotyping of Tfam Conditional Knockout Mice
Respiratory chain dysfunction is an important contributor to human pathology (1 –3 ). The generation of animal models has much facilitated in-depth studies of pathogenetic mechanisms in mitochondrial disease (4 ). The function
耐量异常。PPAR- 激动剂 WY14、643治疗可纠正MKR小鼠糖代谢异常。（二）MODY2动物模型zhang等利用基因敲除技术制得肝脏葡萄糖激酶(GCK) -小鼠，发现随着时间延长，该鼠血糖逐渐升高，糖耐量减退，6周龄小鼠空腹血糖显著高于对照组小鼠，这种肝细胞GCK活性减退所引起的疾病与人MODY相似，可作为MODY的动物模型。（三）线粒体糖尿病Silva等发现β细胞Tfam(mitochondrial transcriptionfactor A)突变的小鼠，大约5周龄时发生糖尿病，表现为严重mtDNA耗竭
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