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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.116 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人糖原磷酸化酶M(PYGM)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human PYGM(Glycogen Phosphorylase, Muscle) ELISA Kit |
| 别名 | GPMM; Phosphorylase,Glycogen; McArdle Syndrome,Glycogen Storage Disease Type V; Myophosphorylase |
| 货号 | ELK1575 |
| 反应种属 | Human |
| P11217 | P11217 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.116 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PYGM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PYGM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PYGM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PYGM in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase;Metabolic pathway;Endocrinology; |

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文献和实验相关实验
Mol Cell:黄波团队揭示糖原代谢调控记忆性 T 细胞早期应答反应
途径产生 NADPH,NADPH 维持细胞内氧化还原稳态,保证 Tm 细胞二次激活反应时的高质量和高活性。 糖原磷酸化酶(Glycogen phosphorylase,PYG)是糖原分解反应的关键酶,催化糖原裂解为 1 磷酸葡萄糖,具有三种亚型(PYGL,Liver;PYGM, Muscle;PYGB, Brain)。本研究发现 PYGB 在 T 细胞中高表达。 重要的是,PYG 一直被认为通过其丝氨酸磷酸化而发挥活性功能;本研究还首次发现 TCR 的下游信号分子 ZAP70/LCK 能够
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人糖原磷酸化酶M(PYGM)酶联免疫吸附检测试剂盒【Human PYGM(Glycogen Phosphorylase, Muscle) ELISA Kit】
¥1820 - 2600





