- ￥1820 - 2600
- ELK Biotechnology
- 1.56-100 ng/mL
- 0.46 ng/mL
- 武汉
- ELK10355
- 2026年05月21日
企业认证
相关产品推荐更多 >
小鼠镍纹蛋白样蛋白(METRNL)酶联免疫吸附检测试剂盒【Mouse METRNL(Meteorin Like Protein) ELISA Kit】
￥1960
人蛋白酪氨酸磷酸酶受体S(PTPRS)酶联免疫吸附检测试剂盒【Human PTPRS(Protein Tyrosine Phosphatase Receptor Type S) ELISA Kit】
￥1820
大鼠粘蛋白1(MUC1)酶联免疫吸附检测试剂盒【Rat MUC1(Mucin 1) ELISA Kit】
￥1820
大鼠神经细胞黏附分子(NCAM)酶联免疫吸附检测试剂盒【Rat NCAM(Neural Cell Adhesion Molecule) ELISA Kit】
￥1680
人血管非炎性蛋白1(VNN1)微量上样酶联免疫吸附检测试剂盒【Human VNN1(Vanin 1) Microsample ELISA Kit】
￥1820
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉益普生物科有限公司
- 检测范围：
1.56-100 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
tissue homogenates, cell lysates and other biological fluids
- 灵敏度：
0.46 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 人蛋白二硫化物异构酶A3(PDIA3)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human PDIA3(Protein Disulfide Isomerase A3) ELISA Kit |
| 别名 | P58; ERp61; ERp57; ERp60; GRP57; PI-PLC; GRP58; Glucose Regulated Protein,58kDa; Protein Disulfide Isomerase-Associated 3; Endoplasmic reticulum resident protein 57 |
| 货号 | ELK10355 |
| 反应种属 | Human |
| P30101 | P30101 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.46 ng/mL |
| 标准品 | 100 ng/mL |
| 检测范围 | 1.56-100 ng/mL |
| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDIA3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDIA3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDIA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDIA3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Enzyme & Kinase;Metabolic pathway; |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验酶免疫测定（enzyme immunoassay, EIA）或免疫酶技术（immunoenzymatic technique）是指用酶标记抗体或酶标记抗体进行的抗原抗体反应。它采用抗原与抗体的特异反应与酶连接，然后通过酶与底物产生颜色反应，用于定量测定。目前常用的方法称为酶联免疫吸附法（enzyme-linked immunosorbent assay, ELISA），其方法简单，方便讯速，特异性强。ELISA是由抗原（抗体）先结合在固相
The following video tutorial described how to run a sandwich ELISA, also known as enzyme-linked immunosorbent assay, using a general PeproTech protocol. 接下来的实验指导讲述PeproTech夹心法ELISA，即酶联免疫吸附试验的通用步骤 The capture, standard, and detection are supplied
1179380155 请问能用ELISA（酶连接免疫吸附）法检测基因突变吗？感激不尽！ woxingwosu ELISA-based Ki-ras gene mutation analyses in pancreatic and cholangiocarcinoma cells and tissues. Abstract Detection of Ki-ras mutations
技术资料暂无技术资料 索取技术资料
