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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
15.63-1000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
5.4 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1680.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2400.0 |
| 中文名称 | 人粒细胞集落刺激因子(GCSF)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human GCSF(Colony Stimulating Factor 3, Granulocyte) ELISA Kit |
| 别名 | CSF3; G-CSF; Granulocyte Colony Stimulating Factor; Pluripoietin; Filgrastim; Lenograstim |
| 货号 | ELK1025 |
| 反应种属 | Human |
| P09919 | P09919 |
| 检测类型 | Sandwich |
| 灵敏度 | 5.4 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GCSF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GCSF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GCSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GCSF in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Cytokine;Hematology; |

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文献和实验Bacteriophage T4 Capsid Packaging and Unpackaging of DNA and Proteins
downstream processing. Proteins that we have produced by the phage PEPP platform include human HIV-1 protease, micrococcal endonuclease from Staphylococcus aureus , restriction endonuclease Eco RI, luciferase, human granulocyte colony stimulating factor (GCSF
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