大鼠调节激活正常T-细胞表达分泌因子(RANTES)酶联免疫
吸附检测试剂盒【Rat RANTES(Regulated On Activation In Normal T-Cell Expressed And Secreted) ELISA Kit】- ￥1680 - 2400
- ELK Biotechnology
- 0.16-10 ng/mL
- 0.058 ng/mL
- 武汉
- ELK10229
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉益普生物科有限公司
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Rat
- 样本：
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
0.058 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1680.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2400.0 |
| 中文名称 | 大鼠调节激活正常T-细胞表达分泌因子(RANTES)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat RANTES(Regulated On Activation In Normal T-Cell Expressed And Secreted) ELISA Kit |
| 别名 | CCL5; SCYA5; SISd; EoCP; Chemokine C-C-Motif Ligand 5; Eosinophil Chemotactic Cytokine; T-cell Specific Protein p288; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 5 |
| 货号 | ELK10229 |
| 反应种属 | Rat |
| 检测类型 | Sandwich |
| 灵敏度 | 0.058 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat RANTES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat RANTES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat RANTES, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat RANTES in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Cytokine;Tumor immunity;Infection immunity;Immune molecule; |
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文献和实验相关实验
，这些黏附分子被推测与嗜酸性粒细胞阳离子蛋白（ECP）的释放，趋化性以及嗜酸性粒细胞的穿透转移相关。而这三种现象由多种细胞因子介导并增强，包括干扰素γ、肿瘤坏死因子α（TNFα）和白细胞介素1（IL-1）；趋化因子包括调节激活正常T细胞表达和分泌因子（RANTES）、IL8、MCP-1和巨噬细胞炎症蛋白-1（MIP-1）。这类炎性细胞因子激活细胞间信号级联反应包括MAPK和NF-κB信号转导途径。 活化T细胞的产物中嗜酸性粒细胞激活细胞因子（IL5，IL3，GMCSF）是哮喘发病的基础，已有研究显示
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大鼠调节激活正常T-细胞表达分泌因子(RANTES)酶联免疫吸附检测试剂盒【Rat RANTES(Regulated On Activation In Normal T-Cell Expressed And Secreted) ELISA Kit】
￥1680 - 2400
