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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
62.5-4000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
plasma
- 灵敏度:
26 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 大鼠凝血因子Ⅻ(F12)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat F12(Coagulation Factor XII) Microsample ELISA Kit |
| 别名 | FXII; HAF; Contact Factor; Hageman Factor |
| 货号 | ELK10027MS |
| 反应种属 | Rat |
| D3ZTE0 | D3ZTE0 |
| 检测类型 | Sandwich |
| 灵敏度 | 26 pg/mL |
| 标准品 | 4000 pg/mL |
| 检测范围 | 62.5-4000 pg/mL |
| 样本类型 | plasma |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat F12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat F12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat F12 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Metabolic pathway;Hematology; |

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文献和实验Evaluation of Proliferation of Neural Stem Cells In Vitro and In Vivo
) SVZ culture medium (see recipe ) Dulbecco's Modified Eagle's Medium:F‐12 nutrient mixture (DMEM/F12) with GlutaMAX‐I (Invitrogen, cat. no. 10565‐018; optional
培养的各种培养基内都含有必需氨基酸。 2、单糖:培养中的细胞可以进行有氧与无氧酵解,六碳糖是主要能源。此外六碳糖也是合成某些氨基酸、脂肪、核酸的原料。细胞对葡萄糖的吸收能力最高,半乳糖最低。 体外培养动物细胞时,几乎所有的培养基或培养液中都以葡萄糖作为必含的能源物质。 3、维生素:主要扮演辅酶、辅基的角色,必不可少。生物素、叶酸、烟酰胺、泛酸、吡哆醇、核黄素、硫胺素、维生素B12都是培养基常有的成分。 4、无机离子与微量元素:细胞生长除需要钠、钾、钙、镁、氮和磷等基本元素,还需要微量
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