- $1268 - 9764
- abinScience
- France
- FB840012
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
twelve months
- 英文名：
Recombinant Cat NT-ProBNP/NPPB Protein, N-GST
- 供应商：
武汉益普生物科技有限公司
- 规格：
50μg/100μg/1mg
| 规格： | 50μg | 产品价格： | $1268.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | $1868.0 |
| 规格： | 1mg | 产品价格： | $9764.0 |
| 产品名 | Recombinant Cat NT-ProBNP/NPPB Protein, N-GST |
| 货号 | FB840012 |
| 别名 | Natriuretic peptides B, Brain natriuretic factor prohormone, preproBNP, proBNP, Gamma-brain natriuretic peptide, Iso-ANP, Brain natriuretic peptide 35, BNP-35, Brain natriuretic peptide 29, BNP-29, NPPB, BNP |
| 表达系统 | E. coli |
| Accession号 | Q9GLK4 |
| 种属 | Felis catus (Cat) (Felis silvestris catus) |
| 背景 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 蛋白长度 | His27-Arg98 |
| 预测分子量 | 34.52 kDa |
| 性质 | Recombinant |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 说明 | For research use only. |
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文献和实验GST Fusion Protein Preparation
CLEAVAGEAlternate Mini PrepThaw out 1 ml aliquot of fusion protein lysate.Centrifuge to remove precipitate.Incubate 1 ml lysate with 0.4 ml 50% GST beads.Incubate at 4℃ with mixing for 1 hour.Centrifuge 5K 2' in 1.5 ml eppendorf tubes.Wash the beads 4X with PBST
Thaw out 1 ml aliquot of fusion protein lysate. Centrifuge to remove precipitate. Incubate 1 ml lysate with 0.4 ml 50% GST beads. Incubate at 4C with mixing for 1 hour. Centrifuge 5K 2' in 1.5 ml eppendorf tubes. Wash the beads 4X with PBST
Thrombin Cleavage of GST-Fusion protein
to cleave 1 mg of fusion protein at room temperature overnight. 1 X PBS buffer: 140mM NaCl; 2.7mM KCL; 10mM Na2HPO4; 1.8mM KH2PO4; pH 7.3 Thrombin Cleavage of Free Eluted Fusion protein First you have to calibrate amount of thrombin, temperature
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