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- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12 months
- 英文名:
Recombinant Rice ABI5 Protein, N-His
- 供应商:
武汉益普生物科技有限公司
- 规格:
50μg/100μg/1mg
| 规格: | 50μg | 产品价格: | ¥1268.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥1868.0 |
| 规格: | 1mg | 产品价格: | ¥9764.0 |
| 产品名称 | Recombinant Rice ABI5 Protein, N-His |
| 货号 | PR141012 |
| 描述 | Recombinant Rice ABI5 Protein, N-His ( PR141012 ) expressed in E. coli , spanning Met1-Trp388 . Purity: >90% by SDS-PAGE. Highlights His-Tagged — N-terminal 6×His tag for IMAC purification. E. coli Expression — High-yield, cost-effective production. High Purity — >90% purity verified by SDS-PAGE. |
| 应用实验 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 宿主/表达体系 | E. coli |
| 基因号 | Q8RZ35 |
| 状态 | Lyophilized |
| 蛋白质长度 | Met1-Trp388 |
| 分子量 | 43.64 kDa |
| 纯度 | >90% as determined by SDS-PAGE. |
| 储存温度 | -20℃ |
| 别名 | bZIP transcription factor ABI5 homolog, OsABI5, bZIP transcription factor 10, OsBZIP10, ABI5, BZIP10, OREB1, Os01g0859300, LOC_Os01g64000, OsJ_04142, P0489B03.11, P0679C12.1 |

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文献和实验A mutated rice acetolactate synthase (mALS ) gene expressed under the control of the rice callus-specific promoter (CSP) (CSP:mALS ) becomes a useful selectable marker for producing transgenic rice seed with higher accumulation of recombinant
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal
, and usually final protein yield between 0.1 and 2 mg/g cells is acceptable. Another concern is protein degradation. Especially with Pichia , protease activity during cell lysis and purification is always an issue. The importance of N-terminal degradation
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