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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
冷冻
- 保质期:
12个月
- 库存:
99
- 供应商:
上海圻明生物
- 规格:
μg
产品名称:NGF/Beta-NGF活性蛋白, Tag free,Mouse
规格:50μg/100μg
纯度:>90% as determined by SDS-PAGE.
用途:SDS-PAGE,WB,ELISA,Immunogen
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined
by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no
obvious degradation and precipitation were observed. The loss rate is less than 5% within the
expiration date under appropriate storage condition.
重组蛋白生产中应用广泛。蛋白标签是一种方便有效的工具,可以提高重组蛋白的溶解性和稳定性;便于目的蛋白的检测、示踪和纯化。
随着研究的需求和技术的发展,各种标签应运而生,多样化的标签可以满足不同的应用场景。许多重组蛋白在生产过程中溶解度较差,
可以使用融合标签以增加溶解度,融合标签可用于目的蛋白没有特异性抗体的多种应用,如Western Blot、免疫沉淀、ELISA等。
蛋白标签类型主要分为三类,适用于不同的应用场景:表位标签、亲和标签和荧光标签。
- 表位标签往往是短肽序列,可用于免疫学应用,如 Western Blot 和免疫共沉淀。最常用的表位标签有His、FLAG、HA等。
- 亲和标签一般较长,可增加蛋白溶解度,广泛应用于重组蛋白的纯化,如SUMO、Trx、MBP等。
- 荧光标签可用于活细胞和死细胞检测,最常用的荧光蛋白包括绿色荧光蛋白(GFP)、橙色荧光蛋白(OFP)、红色荧光蛋白(RFP)和黄色荧光蛋白(YFP)。它们被广泛用于影像学研究,如细胞定位和共表达实验。
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本公司产品仅供科研使用,不用于临床或其他诊断治疗用途。
欢迎新老客户垂询订购:NGF/Beta-NGF活性蛋白, Tag free,Mouse000
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文献和实验Phos-tag Affinity Electrophoresis for Protein Kinase Profiling
. This article discusses applications of label-free kinase activity profiling by the Phos-tag SDS-PAGE method in the analysis of phosphorylated substrates derived from various kinase reactions. The resolving power of the affinity electrophoresis
, and genotype for each mouse that is tagged and tailed. Keep the log sheets with the pedigree. 4. Together, the pedigree, mating cards, and log sheets should provide the following information for each mouse: birth date, sex, ear tag number, coat color, DNA
Digital Gene Expression by Tag Sequencing on the Illumina Genome Analyzer
a protocol for performing digital gene expression profiling on the Illumina Genome Analyzer sequencing platform. Tag sequencing (Tag?seq) is an implementation of the LongSAGE protocol on the Illumina sequencing platform that increases utility while reducing
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