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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
广州市左克生物
- CAS号:
4289-98-9
- 规格:
10mM*1mL/100mg
| 规格: | 10mM*1mL | 产品价格: | ¥467.5 |
|---|---|---|---|
| 规格: | 100mg | 产品价格: | ¥425.0 |
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文献和实验Problems in bisulfite sequencing reproducibility-DNA Met
I am having huge problems with bisulfite PCR.Southern analysis indicates that my DNA is unmethylated.My first bisulfite treatment also indicated that the DNA was unmethylated.Subsequent treatments have not been able to reproduce this result
Cloning of small RNAs with 5’ phosphate and 3’ OH ends
Introduction The following protocol describes a procedure for the purification and cloning of miRNAs and other small RNAs in the 20-30 nucleotide size range from plant tissue. Figure 1 gives an overview of the cloning procedure
with digoxigenin are bound to the 3′ OH DNA ends by reacting with the TdT enzyme in ultrathin sections on a nickel grid. After incubation with an anti-digoxigenin antibody conjugated with 10-nm colloidal gold, the sections are then stained with uranyl acetate
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