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文献和实验in the tube for 5 minutes to warm to room temperature. 3) Prepare fluorescent reactive dye according to kit instructions included in the LIVE/DEAD® Fixable Dead Cell Stain Kit. For optimal performance of ArC™ reactive beads, always use freshly
to room temperature. 3) Prepare fluorescent reactive dye according to kit instructions included in the LIVE/DEAD® Fixable Dead Cell Stain Kit. For optimal performance of ArC™ reactive beads, always use freshly prepared amine-reactive dye
Fastfilter Plasmid Maxi Kit Spin Protocol
is recommended. It is important to dilute the bacterial culture (10 to 20 fold) to enable photometric measurement in the linear range between 0.1 and 0.5 OD600. For maximal yields, the OD600 of cultures should be under 3.0. If using a frozen glycerol stock
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