Rabbit anti-VAP-33 Antibody, A

【求助】抗体名称Ab-33是什么意思？

: 这个抗体是非磷酸化的抗体non-phospho-antibody ，33 代表的是基因位点 感觉不应该说“33 代表的是基因位点”，准确的说是指：该抗体的免疫原是合成非磷酸化的β-Catenin的33位丝氨酸附近的多肽。 Anti β-Catenin (Ab-33) Immunogen：Synthesized non-phosphopeptide derived from human β-Catenin

【讨论】抗体和那些不能说的"秘密"-抗体选择的PASS标准 (请加精)

专家观点 哥本哈根大学Dr.John Couchman认为优质抗体，尤其是多抗，应该是2步纯化： Not only must I affinity purify the antibody but also perform antigen adsorption on my tissues sections. Unfortunately, many commercial antibodies are not affinity purified from serum

IMMUNOSCREENING OF WITH ANTIBODY PROBES

in 10mM IPTG (MW=238.31). Briefly blot the excess solution from the filters, and wrap them in cellophane. These should be moist and stored at 4°C. We typically adsorb serum with five 150 mm Petri dishes of lysed E. coli to reduce anti-E. coli antibody