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文献和实验Ampicillin (50mg/ml) Ampicillin Dissolve Ampicillin (Na salt from Sigma, A-9518) in H2 0. Store 1 ml aliquots at 20o C in common freezer for stock solutions 上一篇:PREPARATION
In Situ Hybridization Using Digoxigenin Labeled Probes
and the attached fat body. If this does not occur, carefully dissect away the fat body 5) Transfer the "fat free" glands into the fixative and incubate for 4-5 minutes 6) Pick up the coverslip and glands with a clean slide by carefully touching the slide
Human FastTrack mRNA Isolation
immersion and homogenization in ddH2 O and ethanol 3 times sequentially, may require boiling H2 O, SDS, or 10% bleach in addition. An RNAse degrader such as RNAse Zap can also be used. 3.Shear each sample with a 3-5 second run of the tissue homogenizer
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