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文献和实验Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles
and Analysis ). It may be necessary to include exogenous lipid during the solubilization step to stabilize the protein (2 ). Ideally, the chosen detergent should have a high critical micelle concentration to enable the detergent to be removed following
Fluorescence Quenching Methods to Study Lipid‐Protein Interactions
25 mg/ml di(9,10‐dibromostearoyl)phosphatidylcholine (BrPC; protocol 3 ) in chloroform Oxygen‐free nitrogen
Measurement of Oxygen Radicals and Lipid Peroxidation in Neural Tissues
) 10 mM HCl, ice cold Oxidized glutathione (GSSG) 5 mg/ml OPA solution (see recipe
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