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文献和实验Analysis of the Role of the AMP-Activated Protein Kinase in the Response to Cellular Stress
(1 ,2 ). The kinase is activated by phosphorylation by an upstream protein kinase termed AMP-activated protein kinase kinase (AMPKK) (3 ). Elevation of 5’-AMP activates the cascade by a complex mechanism involving binding of the nucleotide to both the upstream kinase
Introduction to the EMSA (Gel Shift) Technique
. The ability to resolve protein:DNA complexes depends largely upon the stability of the complex during the brief time (approximately one minute) it is migrating into the gel. Sequence-specific interactions are transient and are stabilized
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
:chloroform:isoamyl alcohol, UltraPure (25:24:1 v/v/v; Invitrogen) Phosphate-buffered saline (PBS; GIBCO 20012) Protease inhibitor cocktail (Sigma P8340) Proteinase K (10 mg/mL in TE buffer, pH 8.0; Invitrogen) PureLink HQ 96 Mini
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