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- 2026年04月15日
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文献和实验Materials2 ml size Sarstedt screw cap tubesBIO-RAD Disposable Chromatography Column （BIO-RAD cat. #732-6008）Oligo dT celluloseRNase-free water5.0 M NaCl0.5 M EDTA1.0 M Tris, pH 7.410% SDS70% EtOHBefore starting protocol1. Measure out 75 mg of oligo
and IPTG directly to the agar before pouring the plates.Here’s a recipe that works for xgal/IPTG/amp plates: 1 liter 2XYT with agar Cool to 60 ℃ Add 1 ml stock amp (100 mg/ml)5 ml xgal (20 mg/ml in DMF-- store in the dark by wrapping
Kamps's Western Blotting Protocol
antibodies,longer washes reduce the signal,shorter washes increase the background. 8)Incubate the filter for 30 min with 125I-protein A in 3% BSA in rinse buffer (You can re-use your blocking buffer for this).We use ICN's 68038 (approx.30mCi/mg specific
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