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文献和实验Assembly of Nucleosomal Templates by Salt Dialysis
Basic Protocol 1: Assembly of Nucleosomal Templates by Step Salt Dialysis Materials ∼1 to 2 mg/ml sonicated calf thymus DNA (∼
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
-galactopyranoside (X-gal; 25 mg/mL in DMF [dimethylformamide]) Agarose gel (1%, UltraPure; Invitrogen) Antibody, specific for the protein of interest Bead eluting buffer Bovine serum albumin (BSA; 0.5% in PBS; New England Biolabs) Cell
, Tris, TWEEN-20, Folin Ciocaltew Reagent, sodium dodecyl sulphate (SDS), NaTartrate, NaOH, NaCO3, bovine serum albumin, HCl, protein sepharose A, butanol, CuSO4,, 10x stock Trypsin/EDTA solution, tetramethylethylenediamine (TEMED), ammonium persulphate
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