Thermo Scientific (Alfa Aesar)
赛默飞实验室化学品试剂J62157.ABAgarose Gel Loading Dye (6X, Alkaline)10ml- ￥1566
- Thermo Fisher
- J62157.AB
- 2026年04月15日
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文献和实验Agarose Gel Electrophoresis for the Separation of DNA Fragments
and Separation of DNA Fragments 1) Add loading dye to the DNA samples to be separated (Fig. 2). Gel loading dye is typically made at 6X concentration (0.25% bromphenol blue, 0.25% xylene cyanol, 30% glycerol). Loading dye helps to track how far
, 1 mL 6X loading dye 1. Start with 3 uL of PCR reactions in PCR plates, after remainder is transferred to U-bottom plates (see next section). 2. Pour gel with four combs of 26 wells
REACTIONS (quality control) Agarose gel: 1% agarose, 1X TAE, 0.5 mg/mL ethidium bromide Buffer: 1X TAE, 0.5 mg/mL ethidium bromide Loading dye (6X): 15% Ficoll-400, 0.25
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