Thermo Scientific (Alfa Aesar)
赛默飞实验室化学品试剂045598.KS氮化硼矩形盘;长 (mm), 125;宽 (mm),125;厚 (mm), 12.71each- ￥10886
- Thermo Fisher
- 045598.KS
- 2026年04月15日
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文献和实验.Repeat steps 11. and 12. This is a total of two washes with 50 mM MES, pH 5.0.14.Remove the supernatant and resuspend the activated and washed microspheres in 100 μL of 50 mM MES, pH 5.0 by vortex and sonication for approximately 20 seconds.15.Add 125, 25
in 100 μL of 50 mM MES, pH 5.0 by vortex and sonication for approximately 20 seconds. 17.Add 125, 25, 5 or 1 μg protein to the resuspended microspheres. (Note: We recommend titration in the 1 to 125 μg range to determine the optimal
Preparation of Chromosome-sized Yeast DNA Molecules in Solid Agarose 1. Grow 5 ml yeast culture in YPD Broth to " stationary" (OD600 10-14) 2. Transfer 1ml to a microfuge tube ( PGC Scientific 2.2ml tube #509-220) 3. Pellet cells (20 seconds
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