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文献和实验Automated Protein NMR Structure Determination in Solution
The main drawback of protein NMR spectroscopy today is still the extensive amount of time required for solving a single structure. The main bottleneck in this respect is the manual evaluation of the experimental spectra. A clear solution
Microscale thermophoresis (MST) is a new method that enables the quantitative analysis of molecular interactions in solution at the microliter scale. The technique is based on the thermophoresis of molecules, which provides information
b-galactosidase Solution Assay
of 0.25 M Tris HCl pH 8.0. • Freeze thaw the cells 3 times by alternating between dry ice and 37°. • Spin for 15 minutes at 4° and transfer the supernatant to a new tube and hold on ice. • Mix the following reaction solution per sample: 264
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