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文献和实验Immunohistochemistry: Fluorescence Protocol 2.0
activation of Ficoll separated PBMNC. Both monocytes and lymphocytes will be activated. The cells are co-cultivated with PMA (1 ng/mL), ionomycin (500 nM/mL) and harvested after 4 hours. After 4 hours, one can expect to see IL-1a, IL-1b, IL-1ra, IL-2, IL
In Vivo Tumor Formation From Primate Embryonic Stem Cells
To achieve human embryonic stem (ES) cell-based transplantation therapies, allogeneic transplantation models of nonhuman primates would be particularly useful. In this chapter, we describe an example of this model. We prepared cynomolgus ES
Use of Simian Immunodeficiency Virus Vectors for Simian Embryonic Stem Cells
immunodeficiency virus-based lentivirus vector. When cynomolgus ES cells are transduced with a simian immunodeficiency virus vector encoding the green fluorescent protein (GFP) gene, a large fraction of cells (greater than 50%) fluoresce, and high levels of GFP
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