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文献和实验Transient Production of scFv-Fc Fusion Proteins in Mammalian Cells
cells HEK293T and suspension HEK293-6E cells. More than 10 �g/mL yield of scFv-Fc protein can be obtained every day for one to two weeks by transient production in HEK293T cells, whereas soluble HEK293-6E cells produce more than 100 �g/mL scFv-Fc protein
Recombinant fusion proteins incorporating experimental protein domains fused to immunoglobulin Fc regions have become widely utilized in studies of protein–ligand interactions. The advantages of these systems include an inherent increase
Immortalised cultured cells are powerful tools to assess the function of ectopically expressed proteins. However, it must be ensured that the protein of interest is functional in the host system and display native behaviour. In particular
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