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文献和实验1 in the human embryonic kidney (HEK293) cell line are introduced. In isolated cell culture mitochondria, the proton leak can be measured and modulators of UCP1 activity can be tested. As mouse UCP1 in this system shows native behaviour, this may be a suitable
, as illustrated by the homodimerization of the CXCR4 receptor and the recruitment of ??arrestin2 to agonist?activated G?protein?coupled receptors. This noninvasive and homogeneous assay provides a robust and sensitive proteomic platform with applications for basic
A Protocol for Heterologous Expression and Functional Assay for Mouse Pheromone Receptors
of G protein-coupled receptors, vomeronasal receptor type 2 (V2Rs), expressed in the pheromone-detecting vomeronasal organ (VNO) (Chamero et al., Nature 450:899–902, 2007; Haga et al., Nature 466:118–122, 2010; Kimoto et al., Curr Biol 17:1879–1884
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