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文献和实验Protocol for anti-HA antibody Western Blotting
seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes again(in 1XPBS
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
for at least 2 hours at 4 °C with rocking. BMblock-mouse antibody buffer 2.5ml needed for each sample: 10% Goat serum (heat inactivated 30 min at 56 °C) 1% boehringer blocking reagent in PBSw Heat the mixture at 65 °C
Studying Wound Repair in the Mouse
2 , or protocol 3 Normal goat serum (NGS; Vector Laboratories, cat. no. S1000) Anti‐cytokeratin 6 antibody (Abcam, cat. no. ab24646
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