Alexa Fluor® 488 Mouse Anti-Hu

AbC Anti-Mouse Bead Kit

together to calculate compensation in multicolor immunophenotyping experiments that incorporate a LIVE/DEAD® fixable dye by following the protocol outlined below: 1)        Gently vortex the ArC™ Amine Reactive Beads Kit and the AbC™ Anti-Mouse Bead Kit

secondary antibody review -- data from 99 publications

cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey

Vybrant® DyeCycle Green and Orange Stains

degradation. Incubate at 37?C for 30 minutes, protected from light. For cells stained with Vybrant® DyeCycle™ Green stain, analyze samples on flow cytometer using 488 nm excitation and green emission (Figure 3). For cells stained with Vybrant®