MIS RII mFc Chimera Protein, H

Generating NK Cell Receptor-Fc Chimera Proteins from 293T Cells and Considerations of Appropriate Glycosylation

cells. We present here (i) a detailed protocol for the production and purification of soluble recombinant NK cell receptors tagged with human IgG1-Fc (thus termed receptor-Fc chimera or receptor-Ig fusion protein) and (ii) a protocol for cell staining

Antisense Oligonucleotides Targeting RI Subunit of Protein Kinase A: In Vitro and In Vivo Anti-Tumor Activity and Pharmacokinetics

cAMP-dependent protein kinase (PKA) is involved in various cellular functions such as cell proliferation, gene induction, and metabolism (1 ,2 ) and its regulatory subunits have been suggested as a drug target for cancer and other diseases

Cell-Adhesion Assays: Fabrication of an E-Cadherin Substratum and Isolation of Lateral and Basal Membrane Patches

with purified proteins. The methods described here overcome these problems. Using a mammalian expression system, a chimeric protein comprising the extracellular domain of E-cadherin fused at its C-terminus to the Fc domain of human IgG1 (E-cadherin∶Fc