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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
-20°C
- 克隆性:
单克隆
- 适应物种:
Human
- 保质期:
12 months
- 级别:
一抗
- 供应商:
武汉研升生物科技有限公司
- 宿主:
Human
- 应用范围:
ELISA, Bioactivity: FACS, Functional assay, Research in vivo
- 抗体英文名:
Research Grade Anti-Human CD4 (M-T413)
- 规格:
100μg/1mg
| 规格: | 100μg | 产品价格: | ¥2588.0 |
|---|---|---|---|
| 规格: | 1mg | 产品价格: | ¥7788.0 |
| 产品名 | Research Grade Anti-Human CD4 (M-T413) |
| 货号 | HF998096 |
| 别名 | M-T413, MT413, MT413 |
| 表达系统 | Mammalian Cells |
| 靶标 | T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, CD4 |
| Accession号 | P01730 |
| 宿主 | Human |
| 种属反应性 | Human |
| 克隆性 | Monoclonal |
| 克隆号 | M-T413 |
| 同种型 | IgG |
| 内毒素水平 | Please contact the lab for this information. |
| 纯度 | >95% purity as determined by SDS-PAGE. |
| 应用 | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| 纯化方式 | Protein A/G purified from cell culture supernatant. |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C. |
| 说明 | For research use only. Not suitable for clinical or therapeutic use. |

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文献和实验1. Treat cells. For PC12 or NIH 3T3 cells a moderately confluent 60 mm plate is used. 2. Aspirate media. Rinse cells with PBS, aspirate.3. Lyse cells in 200 uL of SDS sample buffer (100 ℃). Immediately scrape the cells off the plate with a rubber
Inhibition of HIV Infection by Lectin Binding to CD4
target to block infection. One of the first events in the infection of T-cells by the human immunodeficiency virus (HIV) is the binding of the viral envelope glycoprotein gp120 to the differentiation antigen CD4 expressed on the host cell
and for M.W. determination. After transfer, rinse the blot 2 times with TBST (10 mM Tris (pH 7.4), 150 mM NaCl, 0.05% Tween-20). Block with 4% BSA in TBST for 45 - 60 minutes. I use RIA grade BSA from USB
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