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文献和实验下放在玻片上观察。 · Indicator cells: Vero (African green monkey kidney, ATCC CCL-81 or CCRC 60013) or 3T6(mouse fibroblast, ATCC CCL-96 or CCRC 60070)，细胞须先测试无污染。αMEM with 10%FBS ( medium for Vero cell) 配制试剂: · 无菌HBSS︰配制Hanks’ balanced salt solution，以0.22mm无菌
micron pore size filter. The pouch containing Leibovitz L-15 Media powder for 1 liter, catalog #LK003250 is $18. Sterile Earle's Balanced Salt Solution (EBSS) with calcium, magnesium, bicarbonate and phenol red is supplied with the PDS (Papain
TISSUE FIBROBLAST CULTURES FOR CHROMOSOME ANALYSIS
as the harvest. IV. Slide Preparation: See Regular Procedure for Culturing Lymphocytes. V. Solutions: Colcemid working solution: 10 mcg/ml Colcemid in Hank's Balanced Salt Solution, store at 4 C. Complete Nutrient Mix F-10: 100 ml Nutrient
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