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- TD-AB2008
- 2026年04月08日
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- 技术资料
- 形态：
Liquid
- 保存条件：
Store at 2-8°C under sterile conditions.
- 供应商：
武汉天德
- 抗体英文名：
Alexa Fluor® 790 Goat Anti-Mouse IgG (H+L)
- 规格：
50ul
| Product | Alexa Fluor® 790 Goat Anti-Mouse IgG (H+L) |
|---|---|
| Code Number | TD-AB2008 |
| Conjugate | Alexa Fluor® 790 |
| Physical State | Liquid |
| Size | 20ul/50ul/100ul |
| Antibody Concentration | 1.5 mg/ml |
| Suggested Dilution Range | 1:50,000 - 1:200,000 for Western blotting |
| Fluorophore | Alexa Fluor®790 carboxylic acidλmax=792 nm; Emax=803 nm |
| Fluorophore/Protein Absorbance Ratio | A784/A280 = 1.2 |
| Buffer | 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 |
| Stabilizer | 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) |
| Preservative | 0.05% Sodium Azide |
| Storage | Store at 2-8°C under sterile conditions. |
| Purity | Affinity chromatography with Protein A/G |
| Background | Immunoglobulin G (IgG) is one of the most abundant proteins in serum with normal levels between 9.5-12.5 mg/ml in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10¹¹ variants. |
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
偶联物，最大限度的提高了信噪比。图2. CF染料的超强特异性；由于AF染料制备过程中含有大量带负电的磺化基团，容易非特异性结合正电基团，请看AF790 Dye与CF790的条带特异性对比。3. 优异的标记效率生物偶联后的活性染料一般很容易水解，会在运输，操作及储存中带来诸多不便，最终致染料结合效率低下。像Alexa Fluor® dyes,DyLight? dyes and IRDyes®等严重的磺化作用有较强的吸湿性，恶化水解作用等问题。例如： AlexaFluor® 488 在应用时琥珀酰亚胺脂酯
'）2 Fragment（Alexa Fluor® 555 Conjugate）#4413 Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 647 Conjugate）#4414 抗小鼠： Anti-Mouse IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4408 Anti-Mouse IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor
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