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文献和实验on safety and efficacy. Therefore, assessment of immunogenicity of the therapeutic protein is an essential part of its clinical development. This involves testing strategies which provide relevant information on antibody responses. This chapter describes
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
Non-fluorescent protein/RNA double-labeling
, 50 ug/ml heparin, and 250 ug/ml tRNA). You can also include 1% BSA providing you know it is ultra pure/RNase-free. With our reagents, we find we get similar results without it. 4. Incubate with the primary antibody (diluted appropriately in PBTH
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