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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
- 英文名:
Recombinant Human APLNR/APJ Protein, N-GST & C-His
- 库存:
999
- 供应商:
abinscience
- 规格:
50ug/100ug/1mg
| 规格: | 50ug | 产品价格: | ¥211.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥311.0 |
| 规格: | 1mg | 产品价格: | ¥1627.0 |

| Product name | Recombinant Human APLNR/APJ Protein, N-GST & C-His |
|---|---|
| Catalog No. | HW667012 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Accession | P35414 |
| Protein length | Glu2-Ser26&Leu167-Gly200&Tyr309-Asp380 |
| Nature | Recombinant |
| Predicted molecular weight | 42.91 kDa |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative Names | AGTRL1, APJ, APLNR, Angiotensin receptor-like 1, Apelin receptor, G-protein coupled receptor APJ, G-protein coupled receptor HG11 |
| Species | Homo sapiens (Human) |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验depends on product and its function. N-terminal sequencing revealed that the purified rBoNTC(Hc )-N is missing the first eight amino acids of the N-terminus of the protein, whereas the purified rBoNTC(Hc )-C protein is intact. After a mouse bioassay test
Mutational Analysis of the Human Protein C Gene
The single gene for protein C is located at position q13–q14 on chromosome 2 (1 ). Two groups have described human genomic clones of protein C isolated from phage l charon libraries using cDNA for human protein C as hybridization probes
Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
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