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- ChromaDex
- 详询
- ASB-00008075-010
- 2026年04月02日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
无
- 库存：
大量
- 供应商：
上海熹垣生物
- CAS号：
27013-91-8
- 规格：
10mg
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文献和实验LABELING ALPHA-ACTININ WITH IATR
of injection buffer)and reading OD555. D/P = {OD555 x 41 / 60,000} / {(mg/ml)/ 100,000} 13.Dilute to 5 mg/ml for microinjection if necessary.Extra conjugated alpha-actinin can be stored as aliquots in liquid N2.
ALPHA FACTOR ARREST AND RELEASE
Alpha Factor Arrest and Release Alpha factor stock is 10-3 M in 0.1N HCl (i.e. resuspend 5 mg alpha factor in 2.97 ml 0.1N HCl). To arrest BAR1 strains, the pheromone should be diluted to a final concentration
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
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