Anti- FcRH5 / IRTA2 / CD307e R

（共享）ELISA原理与实验方法

, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS

Using Biacore to Measure the Binding Kinetics of an Antibody‐Antigen Interaction

  Figure 19.14.3 Immobilization of anti‐β2µ‐globulin antibody to the chip surface. Each step and corresponding response is labeled at the top of the sensorgram. Each X on the response represents a report point

secondary antibody review -- data from 99 publications

cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey