- ￥6225
- OriGene
- 美国
- TA398168
- 2026年04月01日
- ELISA, IHC, WB
- Donkey
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human IgG whole molecule
- 形态：
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
- 保存条件：
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- 克隆性：
Polyclonal
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
TA398168
- 级别：
科研级
- 库存：
4周
- 供应商：
OriGene
- 宿主：
Donkey
- 应用范围：
ELISA, IHC, WB
- 抗体英文名：
F(ab')2 Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
- 抗体名：
F(ab')2 Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
- 规格：
500 ug
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
dehydrogenases. Progr. Histochem. Cytochem . 4 :225. Berger, J., A.D. Howard, L. Gerber, B.R. Cullen, and S. Udenfriend. 1987. Expression of active, membrane-bound human placental alkaline phosphatase by transfected simian cells. Proc. Natl. Acad. Sci
ELISA Procedure for Measuring Serum Antibody Titer
IgG/alkaline phosphatase conjugate (usually 1:1000 or greater) that is appropriate to the source of your antibodies in PBS-T. Pipette 200 µL of this solution into each of the wells and incubate for three hours at 37 °C or overnight at 2-6 °C
the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody preparation (serum, tissue culture supernatant, or ascites).Apply Binding buffer again ~ 200 ml.Apply Elution buffer ~ 100 ml.Collect 3 ml
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