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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Armenian Hamster IgG whole molecule
- 形态:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
- 保存条件:
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- 克隆性:
Polyclonal
- 适应物种:
Hamster
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
TA398088
- 级别:
科研级
- 库存:
4周
- 供应商:
OriGene
- 宿主:
Goat
- 应用范围:
ELISA, IHC, WB
- 抗体英文名:
Armenian Hamster IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated
- 抗体名:
Armenian Hamster IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated
- 规格:
1 mg
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
ELISA Procedure for Measuring Serum Antibody Titer
IgG/alkaline phosphatase conjugate (usually 1:1000 or greater) that is appropriate to the source of your antibodies in PBS-T. Pipette 200 µL of this solution into each of the wells and incubate for three hours at 37 °C or overnight at 2-6 °C
IMMUNOSCREENING OF WITH ANTIBODY PROBES
. Incubate these membrane sections with secondary antiserum for 1 hour at room temperature (For detecting human antibodies, we use Sigma #A-3150: goat anti-human IgG alkaline phosphatase conjugate, diluted 1:1000) Wash membranes in TBST for 5 minutes
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