- ￥9230
- OriGene
- 美国
- TA389469
- 2026年04月01日
- ELISA, FC
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
1XPBS
- 保存条件：
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized antibodies are shipped at ambient temperature.
- 克隆性：
Monoclonal
- 适应物种：
Human
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
TA389469
- 级别：
科研级
- 库存：
2周
- 供应商：
OriGene
- 宿主：
Rabbit
- 应用范围：
ELISA, FC
- 靶点：
GPRC5D
- 抗体英文名：
Biotinylated Anti-GPRC5D antibody(DM60), Rabbit mAb
- 抗体名：
Biotinylated Anti-GPRC5D antibody(DM60), Rabbit mAb
- 规格：
100 ug
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
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